Effects of CD133 Silencing on Survival and Migration of HT-29 Colorectal Cancer Cells

Authors

  • Abbas Pakdel Department of Biochemistry, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran
  • Afsaneh Faraji Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Ahad Mokhtarzadeh Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Aliakbar Shabani Department of Biotechnology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran|Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
  • Behzad Baradaran Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Milad Asadi Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Morteza Akbari Department of Biotechnology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran|Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran|Semnan Biotechnology Research Center, Semnan University of Medical sciences, Semnan, Iran
  • Navid Shomali Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran|Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
  • Vahid Khaze Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract:

Background: Colorectal cancer (CRC) is attributed as one of the most common malignancies worldwide. CD133 molecule, as a pentaspan transmembrane glycoprotein, confers stem cell-related characteristics, including self-renewal and multi-directional differentiation capability. CD133 plays important roles in the progression of CRC by conferring apoptotic resistance and migration ability. Objective: To investigate the anti-apoptotic and anti-angiogenic effect of CD-133 targeted siRNA in a colorectal cancer cell line. Methods: In this study, CD133-targeted siRNA transfection was conducted into HT-29 cells. MTT assay was employed to evaluate the cytotoxic effects of transfection on the cells. Flow cytometry was used to evaluate the apoptosis rate. The mRNA expression of apoptosis and metastasis related genes were assessed by quantitative Real-Time PCR (qRT-PCR). Wound healing assay was used to assess the migration potency of the infected cells. Results: Expression of CD133 was significantly downregulated after transfection of CD133-specific siRNA. Moreover, the rate of apoptosis was significantly increased after transfection. The migration potential of cells was diminished after transfection. siRNA delivery resulted in the modulation of expression of apoptosis and metastasis-related genes. Conclusion: siRNA mediated targeting of CD133 could be considered as a promising approach to treat CRC through suppressing the cancerous behavior of tumor cells.

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Journal title

volume 16  issue 3

pages  246- 257

publication date 2019-09-25

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